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Home >> Antibodies >> CD15 / FUT4 (Reed-Sternberg Cell Marker)

CD15 / FUT4 (Reed-Sternberg Cell Marker)

Mouse Monoclonal Antibody [Clone SPM119]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Monoclonal Antibody (SPM119).

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FACS analysis of CD15 on human Monocytes using CD15 Monoclonal Antibody (SPM119).

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Immunofluorescence staining of U937 cells using CD15 Monoclonal Antibody (SPM119) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

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Flow Cytometric Analysis of U937 cells using CD15 MAb (SPM119) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

3 Fucosyl N Acetyl Lactosamine; Alpha (1,3) Fucosyltransferase; Alpha 13 fucosyltransferase FucT; ELAM Ligand Fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific; Fucosyltransferase 4; Galactoside 3 L Fucosyltransferase; Lewis X; LeX; SSEA1; Stage Specific Embryonic Antigen 1

Positive Control

U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

U937 histiocytic cell line

Cellular Localization

Cell surface and granular paranuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

~220kDa

Specificity & Comments

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hanjan SN et. al. Clinical Immunology 23(2):172-88

Bioinformatics

  •  Entrez Gene ID: 2526
  •  Gene Symbol: FUT4
  •  SwissProt: P22083
  •  Chromosome Location: 11q21
  •  Unigene: 654379
NBT00277