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Home >> Antibodies >> CD14 (Monocyte / Macrophage Marker)

CD14 (Monocyte / Macrophage Marker)

Mouse Monoclonal Antibody [Clone LPSR/2385]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Lymph Node stained with CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385).

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Formalin-fixed, paraffin-embedded human Tonsil stained with CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385).

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SDS-PAGE Analysis Purified CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of A549 cell lysate using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385).

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Flow Cytometric Analysis of A549 cells using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385).) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385)

Product Details

Synonyms

Lipopolysaccharide receptor; LPS-R; LPSR; Mo2; Monocyte Differentiation Antigen 14; Myeloid cell-specific leucine-rich glycoprotein

Positive Control

A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IHC-P         More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Schumann, R.R. 1992. Function of lipopolysaccharide (LPS)-binding protein (LBP) and CD14, the receptor for LPS/LBP complexes: a short review. Res. Immunol. 143: 11-1
  2. Simmons, D.L., et al. 1989. Monocyte antigen CD14 is a phospholipid anchored membrane protein. Blood 73: 284-289.

Bioinformatics

  •  Entrez Gene ID: 929
  •  Gene Symbol: CD14
  •  SwissProt: P08571
  •  Chromosome Location: 5q31.1
  •  Unigene: 163867
NBT02385