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Home >> Antibodies >> CD137 / 4-1BB / TNFRSF9

CD137 / 4-1BB / TNFRSF9

Mouse Monoclonal Antibody [Clone 4-1BB/3201]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).

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Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).

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Formalin-fixed, paraffin-embedded human spleen stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).

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Formalin-fixed, paraffin-embedded human cerebellum stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).

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Flow Cytometric Analysis of MeOH-fixed HEK293 cells. CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescent staining of MeOH-fixed HEK293 cells. CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201) followed by goat anti-mouse IgG-CF488 (Green). Nuclei are stained with Reddot (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).

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Western Blot Analysis of kidney tissue lysate using CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).

Product Details

Synonyms

4-1BB Ligand Receptor T Cell; Antigen 4-1BB Homolog; CDw137; HLDA VI; Homolog of mouse 4 1BB; induced by lymphocyte activation (ILA); Interleukin activated receptor homolog of mouse Ly63; T-cell antigen 4-1BB / ILA homolog; Tumor necrosis factor receptor superfamily member 9

Positive Control

HEK293 cells, Jurkat whole cell lysate or recombinant human CD137 protein (WB). Human kidney cancer, liver, stomach or thyroid cancer tissue (IHC-P). Expressed on the surface of activated T-cells.

Known Applications & Suggested Dilutions

ELISA, IF, Flow Cyt, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Immunofluorescence (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A recombinant fragment (around aa 19-188) of human CD137 protein (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

32kDa (monomer); 85kDa (dimer)

Specificity & Comments

CD137 belongs to the tumor necrosis factor receptor family and delivers a costimulatory signal to T lymphocytes. It is expressed on activated T cells and binds an inducible ligand that is found on B cells, macrophages and dendritic cells. Interactions between CD137 and its ligand are involved in antigen presentation and the generation of cytotoxic T cells. CD137 antibody may improve cancer treatment, and has been implicated in breast cancer, melanoma and lymphoma.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Zhao S, et al. CD137 ligand is expressed in primary and secondary lymphoid follicles and in B-cell lymphomas: diagnostic and therapeutic implications. Am J Surg Pathol. 2013 Feb;37(2):250-8.

Bioinformatics

  •  Entrez Gene ID: 3604
  •  Gene Symbol: TNFRSF9
  •  SwissProt: Q07011
  •  Chromosome Location: 1p36.23
  •  Unigene: 654459 & 86447