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Home >> Antibodies >> Calpain 1

Calpain 1

Mouse Monoclonal Antibody [Clone CAPN1/1530]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530).

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SDS-PAGE Analysis Purified Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Calcium dependent protease small subunit 1; Calcium-activated neutral proteinase 1; Calpain 1 large subunit; Calpain mu-type; Calpain regulatory subunit; Calpain small subunit 1; Calpain, large polypeptide L1; Calpain-1 catalytic subunit; CANP small subunit; CANP1; CANPL1; CAPN1; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; Mu Calpain

Positive Control

HeLa cells. Skin or Tonsil.

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CAPN1 protein

Cellular Localization

Cytoplasm and Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

80kDa

Specificity & Comments

Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Murachi, T. 1984. Biochem. Soc. Symp. 45: 149-167.

Bioinformatics

  •  Entrez Gene ID: 823
  •  Gene Symbol: CAPN1
  •  SwissProt: P07384
  •  Chromosome Location: 11q13.1
  •  Unigene: 502842
NBT01530