Home >> Antibodies >> Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)

Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)

Recombinant Mouse Monoclonal Antibody [Clone rCALD1/7266]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

Formalin-fixed, paraffin-embedded human smooth muscle stained with h-CALD Recombinant Mouse Monoclonal Antibody (rCALD1/7266). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human smooth muscle stained with h-CALD Recombinant Mouse Monoclonal Antibody (rCALD1/7266). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human smooth muscle stained with h-CALD Recombinant Mouse Monoclonal Antibody (rCALD1/7266). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human smooth muscle stained with h-CALD Recombinant Mouse Monoclonal Antibody (rCALD1/7266). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

CAD; CALD1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; CDM; HCAD; LCAD; NAG22

Positive Control

Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length CALD1 protein

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

150kDa

Specificity & Comments

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.