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Home >> Antibodies >> Bromodeoxyuridine (BrdU)

Bromodeoxyuridine (BrdU)

Mouse Monoclonal Antibody [Clone SPM537]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (SPM537).

Product Details

Synonyms

Bromodeoxyuridine, BUdr

Positive Control

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC] Optimal dilution for a specific application should be determined.

Immunogen

Bromodeoxyuridine (BrdU) conjugated to KLH

Cellular Localization

Nuclear

Species Reactivity

All species

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Depends on the target

Specificity & Comments

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6.
  2. Williams LS et. al. Cytometry, 1990, 11(4):490-7.

Bioinformatics

NBT00494