Home >> Antibodies >> BRG1 / SMARCA4

BRG1 / SMARCA4

Recombinant Rabbit Monoclonal Antibody [Clone BRG1/7633R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human colon stained with BRG1 / Recombinant Rabbit Monoclonal Antibody (BRG1/7633R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human ovarian small cell carcinoma stained with BRG1 Recombinant Rabbit Monoclonal Antibody (BRG1/7633R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human colon stained with BRG1 / Recombinant Rabbit Monoclonal Antibody (BRG1/7633R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human ovarian small cell carcinoma stained with BRG1 Recombinant Rabbit Monoclonal Antibody (BRG1/7633R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

ATP dependent helicase SMARCA4; BAF190A; Brahma protein like 1; BRM/SWI2 related gene 1; global transcription activator snf2l4; Homeotic gene regulator; hSNF2b; Mitotic growth and transcription activator; MRD16; Nuclear protein GRB1; Protein brahma homolog 1; RTPS2; SMARCA4;; SNF2LB; SWI2; Transcription activator BRG1

Positive Control

HeLa or HEK-293T cells. Ubiquitous nuclear expression.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P More Details

ELISA (For coating, order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determin

Immunogen

Recombinant fragment (around aa 200-400) of human SMARCA4 protein (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

185kDa

Specificity & Comments

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2) and Brg-1 (also designated SNF2) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/ SNF, including the NURF (nucleosome remodeling factor).