Home >> Antibodies >> BOB.1 (B-Cell Marker)

BOB.1 (B-Cell Marker)

Mouse Monoclonal Antibody [Clone BOB1/7468]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 Mouse Monoclonal Antibody (BOB1/7468). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 Mouse Monoclonal Antibody (BOB1/7468). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human placenta. Negative tissue control using BOB1/7468 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified BOB1 Mouse Monoclonal Antibody (BOB1/7468). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 Mouse Monoclonal Antibody (BOB1/7468). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human placenta. Negative tissue control using BOB1/7468 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified BOB1 Mouse Monoclonal Antibody (BOB1/7468). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

B cell Oct binding protein 1; B-cell-specific coactivator OBF-1; BOB-1; OBF1; OCA-B; OCAB; OCT-binding factor 1; POU domain class 2-associating factor 1; POU2AF1

Positive Control

Raji or Ramos cells. Spleen or tonsil (IHC).

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG

Mol. Weight of Antigen

35kDa

Specificity & Comments

BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. �Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. �B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.