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Home >> Antibodies >> Biotin (Vitamin B7 or Vitamin H)

Biotin (Vitamin B7 or Vitamin H)

Recombinant Rabbit Monoclonal Antibody [Clone BTN/8672R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human kidney stained with Biotin Rabbit Recombinant Monoclonal antibody (BTN/8672R).HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

Vitamin B7 or Vitamin H

Positive Control

Biotinylated proteins in solution or on tissues

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

In situ Nucleic Acid Hybridization
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Biotinylated sheep immunoglobulin

Cellular Localization

Depends on the target

Species Reactivity

All species

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

244Da

Specificity & Comments

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

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200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

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