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Home >> Antibodies >> Bcl-2 (Apoptosis & Follicular Lymphoma Marker)

Bcl-2 (Apoptosis & Follicular Lymphoma Marker)

Mouse Monoclonal Antibody [Clone SPM117]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western blot analysis of human MCF-7 cell lysate using Bcl-2 Mouse Monoclonal Antibody (SPM117).

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SDS-PAGE Analysis Purified Bcl-2 Mouse Monoclonal Antibody (SPM117). Confirmation of Integrity and Purity of Antibody.

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Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Mouse Monoclonal Antibody (SPM117).

Product Details

Synonyms

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2

Positive Control

MCF-7, Jurkat or HeLa cells. Human tonsil or follicular lymphomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Cellular Localization

Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.

Species Reactivity

Human. Does not react with mouse or rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

25-26kDa

Specificity & Comments

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Pezzella F et. al. American Journal of Pathology, 1990, 137(2):225-32.

Bioinformatics

  •  Entrez Gene ID: 596
  •  Gene Symbol: BCL2
  •  SwissProt: P10415
  •  Chromosome Location: 18q21.33
  •  Unigene: 150749