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Bax (Apoptosis Marker) Antibody [2D2]

In Stock
Catalog Number Formulation Size Price
581-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
581-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
581-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Bax Mouse Monoclonal Antibody (2D2).

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Bax Mouse Monoclonal Antibody (2D2).

Flow Cytometric Analysis of human Jurkat cells using Bax Mouse Monoclonal Antibody (2D2) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).

Flow Cytometric Analysis of human Jurkat cells using Bax Mouse Monoclonal Antibody (2D2) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).

Confocal Immunofluorescence of Human Jurkat cells A. Bax Mouse Monoclonal Antibody (Clone 2D2) B. Goat anti Mouse CF488. labeled with CF488 (Green); Reddot is used to label the nuclei.

Confocal Immunofluorescence of Human Jurkat cells A. Bax Mouse Monoclonal Antibody (Clone 2D2) B. Goat anti Mouse CF488. labeled with CF488 (Green); Reddot is used to label the nuclei.

SDS-PAGE Analysis of Purified Bax Mouse Monoclonal Antibody (2D2). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Bax Mouse Monoclonal Antibody (2D2). Confirmation of Purity and Integrity of Antibody.

Bax Antibody in Western Blot (WB)

Bax Antibody in Western Blot (WB)

Western Blot Analysis of A-549 lysate using Bax Mouse Monoclonal Antibody (2D2).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Monkey
Research Areas
Apoptosis, Autophagy, Breast Cancer, Cancer, Cardiovascular, AKT Signaling, Colon Cancer, Complement System, Lung Cancer, Ovarian Cancer, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Mitochondrion outer membrane
Gene Name
BAX
Positive Control
A-549 cells, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma., Jurkat, K562
Immunogen
A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
Alternate Names
Apoptosis regulator BAX, Bcl-2-like protein 4, Apoptosis regulator BAX cytoplasmic isoform beta, Apoptosis regulator BAX membrane isoform alpha, Bax isoform psi, Bax protein cytoplasmic isoform delta, Bax protein cytoplasmic isoform gamma, Bax zeta, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BCL2L4

Database Links

Entrez Gene ID
581
SwissProt
Q07812

Additional Information

Clone
2D2
Chromosome Location
19q13.33
Mol. Weight of Antigen
21kDa

Related Antibodies

View All BAX Antibodies

Functions

  • Plays a role in the mitochondrial apoptotic process (PubMed:10772918, PubMed:16113678, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816, PubMed:11060313, PubMed:16199525). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed:11060313, PubMed:16199525, PubMed:10772918, PubMed:16113678, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed:11060313, PubMed:16199525, PubMed:10772918, PubMed:16113678, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816).

Key References

  • Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34.
  • Hsu YT, et. al. PNAS, 1997, 94(8):3668-72.
  • Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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