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Home >> Antibodies >> ARF1 (Golgi Apparatus Marker)

ARF1 (Golgi Apparatus Marker)

Mouse Monoclonal Antibody [Clone ARF1/2117]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Kidney stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).

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Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).

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Formalin-fixed, paraffin-embedded human Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).

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Formalin-fixed, paraffin-embedded human Breast stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).

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Formalin-fixed, paraffin-embedded Rat Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).

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SDS-PAGE Analysis Purified ARF1 Mouse Monoclonal Antibody (ARF1/2117). Confirmation of Integrity and Purity of the Antibody.

Product Details

Synonyms

ADP-ribosylation factor 1; ARF1

Positive Control

HeLa cells. Kidney, Liver, Brain or Breast.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human ARF1 protein

Cellular Localization

Cytoplasmic (Golgi Apparatus)

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

20kDa

Specificity & Comments

The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Austin et al. 2000. J Biol Chem. 275(29):21862-9.
  2. Puxeddu et al. 2009. Proc Natl Acad Sci U S A. 106(15):6158-63.

Bioinformatics

  •  Entrez Gene ID: 375
  •  Gene Symbol: ARF1
  •  SwissProt: P84077
  •  Chromosome Location: 1q42.13
  •  Unigene: 286221
NBT02117