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APEX Nuclease I Antibody [CPTC-APEX1-2]

In Stock
Catalog Number Formulation Size Price
328-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
328-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
328-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

Immunofluorescence Analysis of human HeLa cells labeling APEX Nuclease I with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2) followed by Goat anti-Mouse IgG-CF488 (Green). Phalloidin CF640 stains the membrane red.

Immunofluorescence Analysis of human HeLa cells labeling APEX Nuclease I with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2) followed by Goat anti-Mouse IgG-CF488 (Green). Phalloidin CF640 stains the membrane red.

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

Western Blot Analysis of Human A431, A549, PC3, HAP1, HePG2, cell lysate using APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

Western Blot Analysis of Human A431, A549, PC3, HAP1, HePG2, cell lysate using APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

Flow Cytometric Analysis of HeLa cells using APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometric Analysis of HeLa cells using APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

SDS-PAGE Analysis Purified APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis Purified APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2). Confirmation of Purity and Integrity of Antibody.

Analysis of Protein Array containing more than 19,000 full-length human proteins using APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Analysis of Protein Array containing more than 19,000 full-length human proteins using APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

APEX / APE1 is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Patients with genetic variants in APEX1 and XRCC1 have been shown to have a higher risk of lung cancer. Elevated APEX1 levels observed in human testicular cancer may be related to relative resistance to therapy and therefore may serve as a diagnostic marker for refractory disease.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Neuroscience, Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleolus, Nucleus, Nucleus speckle
Gene Name
APEX1
Positive Control
A431, A549, HAP1, HeLa, HePG2, MCF-7, NIH/3T3 and C6 whole cell lysates. Human ovarian carcinoma., PC3
Immunogen
Recombinant human full-length APEX1 protein
Alternate Names
DNA-(apurinic or apyrimidinic site) endonuclease, APEX nuclease, Apurinic-apyrimidinic endonuclease 1, REF-1, Redox factor-1, APEX1; AP endonuclease class I; AP lyase; APE; APE-1; APEN; APEX nuclease; Redox factor-1; APX; protein REF-1

Database Links

Entrez Gene ID
328
SwissProt
P27695

Additional Information

Clone
CPTC-APEX1-2
Chromosome Location
14q11.2
Mol. Weight of Antigen
35kDa

Related Antibodies

View All APEX1 Antibodies

Functions

  • Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.

Key References

  • Robertson, K.A., et al. Cancer Res. 2001.
  • Xuan, W. et al. Anticancer Res. 2005.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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