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Home >> Antibodies >> APEX Nuclease I

APEX Nuclease I

Mouse Monoclonal Antibody [Clone CPTC-APEX1-2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

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Immunofluorescence Analysis of human HeLa cells labeling APEX Nuclease I with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2) followed by Goat anti-Mouse IgG-CF488 (Green). Phalloidin CF640 stains the membrane red.

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

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Western Blot Analysis of Human A431, A549, PC3, HAP1, HePG2, cell lysate using APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

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Flow Cytometric Analysis of HeLa cells using APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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SDS-PAGE Analysis Purified APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).

Product Details

Synonyms

APEX1; AP endonuclease class I; AP lyase; APE; APE-1; APEN; APEX nuclease; Redox factor-1; APX; protein REF-1

Positive Control

A431, A549, PC3, HAP1, HePG2, MCF-7, HeLa, NIH/3T3 and C6 whole cell lysates. Human ovarian carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length APEX1 protein

Cellular Localization

Mitochondrion. Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

35kDa

Specificity & Comments

APEX / APE1 is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Patients with genetic variants in APEX1 and XRCC1 have been shown to have a higher risk of lung cancer. Elevated APEX1 levels observed in human testicular cancer may be related to relative resistance to therapy and therefore may serve as a diagnostic marker for refractory disease.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Robertson, K.A., et al. Cancer Res. 2001.
  2. Xuan, W. et al. Anticancer Res. 2005.

Bioinformatics

  •  Entrez Gene ID: 328
  •  Gene Symbol: PTPN6
  •  SwissProt: P27695
  •  Chromosome Location: 14q11.2
  •  Unigene: 73722
NBT02260