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Home >> Antibodies >> Annexin A1 / (Hairy Cell Leukemia Marker)

Annexin A1 / (Hairy Cell Leukemia Marker)

Mouse Monoclonal Antibody [Clone 6E4/3]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Annexin A1 Mouse Monoclonal Antibody (6E4/3).

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SDS-PAGE Analysis Purified Annexin A1 Mouse Monoclonal Antibody (6E4/3). Confirmation of Integrity and Purity of the Antibody.

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Flow Cytometric Analysis of PFA-fixed HeLa cells. Annexin A1 Mouse Monoclonal Antibody (6E4/3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

ANXA1; ANX1; Annexin-1; Chromobindin-9; LPC1; p35

Positive Control

HeLa, A431, K562 cells; human esophagus, placenta and prostate tissues.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Mixture of native proteins from human bone tissues

Cellular Localization

Nuclear and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

35kDa

Specificity & Comments

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Falini B, Tiacci E, Liso A et al: Simple diagnostic assay for hairy cell leukemia by immunocytochemical detection of annexin A1 (ANXA1). Lancet 363: 1869-1871, Jun 5, 2004. Basso K, Liso A, Tiacci E et al: Gene expression profiling of hairy cell leukemia reveals a phenotype related to memory B-cells with altered expression of chemokine and adhesion receptors. J Exp Med 199:59-68, 2004.

Bioinformatics

  •  Entrez Gene ID: 301
  •  Gene Symbol: ANXA1
  •  SwissProt: P04083
  •  Chromosome Location: 9q21.13
  •  Unigene: 494173
NBT02179