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Home >> Antibodies >> AMACR / p504S (Prostate Cancer Marker)

AMACR / p504S (Prostate Cancer Marker)

Rabbit Monoclonal Antibody [Clone 13H4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human prostate carcinoma stained with AMACR / p504S Rabbit Monoclonal Antibody (13H4).

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Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with AMACR / p504S Rabbit Monoclonal Antibody (13H4)

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IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using 13H4 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Western Blot Analysis of human kidney tissue lysate using AMACR / p504S Rabbit Monoclonal Antibody (13H4).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using AMACR / p504S Rabbit Monoclonal Antibody (13H4).

Product Details

Synonyms

Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM

Positive Control

HEK cells. Human kidney or prostate adenocarcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human AMACR protein

Cellular Localization

Cytoplasm

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

42kDa

Specificity & Comments

This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Xu J et. al. Canc Res. 2000; 60:1677
  2. Jiang Z et. al. Human Pathology. 2003; 34(8):792

Bioinformatics

  •  Entrez Gene ID: 23600
  •  Gene Symbol: AMACR
  •  SwissProt: Q9UHK6
  •  Chromosome Location: 5p13.3
  •  Unigene: 508343