2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker)

Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker)

Mouse Monoclonal Antibody [Clone AAT/6323]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

SDS-PAGE Analysis Purified Alpha-1-Antitrypsin Mouse Monoclonal (AAT/6323). Confirmation of Purity and Integrity of Antibody.

img

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Alpha-1-Antitrypsin Mouse Monoclonal Antibody (AAT/6323). Inset: PBS instead of primary antibody; secondary only negative control.

img

Formalin-fixed, paraffin-embedded human liver in colon stained with Alpha-1-Antitrypsin Mouse Monoclonal (AAT/6323). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using Alpha-1-Antitrypsin Mouse Monoclonal (AAT/6323).

Product Details

Synonyms

A1AT; Alpha-1 protease inhibitor; Alpha-1-antiproteinase (Alpha1AT); Dom1; Serine (or cysteine) proteinase inhibitor clade A member 1; Serpin peptidase inhibitor clade A member 1; Short peptide from AAT (SPAAT); Spi1-1

Positive Control

Human tonsil, hepatocellular carcinoma or histiocytoma (IHC).

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa200-400) of human SERPINA1 protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

46kDa

Specificity & Comments

It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Atkinson, G.W., et al. Ann Clin Lab Sci 3:345-352, 1973.
  2. Kyaw-Myint, T.O., et al. Clin Chem Acta 59:51-54, 1975.

Bioinformatics

  •  Entrez Gene ID: 5265
  •  Gene Symbol: SERPINA1
  •  SwissProt: P01009
  •  Chromosome Location: 14q32.1
  •  Unigene: 525557