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Home >> Antibodies >> Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker)

Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker)

Recombinant Rabbit Monoclonal Antibody [Clone AAT/3167R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified Alpha-1-Antitrypsin Recombinant Rabbit Monoclonal (AAT/3167R). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human Breast stained with Alpha-1-Antitrypsin Recombinant Rabbit Monoclonal Antibody (AAT/3167R).

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Western Blot Analysis of Jurkat and A549 cell lysate using Alpha-1-Antitrypsin Recombinant Rabbit Monoclonal (AAT/3167R).

Product Details

Synonyms

A1AT; Alpha-1 protease inhibitor; Alpha-1-antiproteinase (Alpha1AT); Dom1; Serine (or cysteine) proteinase inhibitor clade A member 1; Serpin peptidase inhibitor clade A member 1; Short peptide from AAT (SPAAT); Spi1-1

Positive Control

Jurkat, A549 or HepG2 cells. Tonsil, hepatocellular carcinoma or histiocytoma (IHC).

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

46kDa

Specificity & Comments

It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Atkinson, G.W., et al. Ann Clin Lab Sci 3:345-352, 1973.
  2. Kyaw-Myint, T.O., et al. Clin Chem Acta 59:51-54, 1975.

Bioinformatics

  •  Entrez Gene ID: 5265
  •  Gene Symbol: SERPINA1
  •  SwissProt: P01009
  •  Chromosome Location: 14q32.1
  •  Unigene: 525557
NBT03167