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Home >> Antibodies >> Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)

Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)

Recombinant Rabbit Monoclonal Antibody [Clone ALPP/2899R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human placenta stained with PLAP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).

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SDS-PAGE Analysis Purified PLAP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of JEG-3 cell lysate using PLAP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).

Product Details

Synonyms

Alkaline phosphatase placental type; Alkaline phosphatase Regan Isozyme; ALP; Alp1; ALPP; Germ-cell alkaline phosphatase; nagao Isozyme; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP-1; PLAP1

Positive Control

JEG-3 or HepG2 cells. Placenta or seminoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human ALPP protein

Cellular Localization

Cytoplasmic and cell surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

70kDa

Specificity & Comments

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wick, MR, et al. 1987;Hum Pathol. 18(9):946-54.

Bioinformatics

  •  Entrez Gene ID: 250
  •  Gene Symbol: ALPP
  •  SwissProt: P05187
  •  Chromosome Location: 2q37.1
  •  Unigene: 284255
NBT02899