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Home >> Antibodies >> AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker)

AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker)

Mouse Monoclonal Antibody [Clone SPM334]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Monoclonal Antibody (SPM334).

Product Details

Synonyms

Alpha fetoglobulin; FETA; HPAFP

Positive Control

Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Alpha feto protein (AFP) Purified from serum of a hepatoma patient

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Monkey. Dog. Pig. Does not react with Cow, Mouse or Rat.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

70kDa

Specificity & Comments

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yazova AK; Goussev AI; Poltoranina VS; Yakimenko EF. Human alpha-fetoprotein epitopes as revealed by monoclonal antibodies. Immunology Letters, 1990 Sep, 25(4):325-30.

Bioinformatics

  •  Entrez Gene ID: 174
  •  Gene Symbol: AFP
  •  SwissProt: P02771
  •  Chromosome Location: 4q13.3
  •  Unigene: 518808
NBT00354