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Beta-Actin (ACTB)

Mouse Monoclonal Antibody [Clone ACTB/1109]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Western blot analysis of Raji cell lysate using Beta Actin / �-Actin Mouse Monoclonal (ACTB/1109).

Formalin-fixed, paraffin-embedded human tonsil stained with Beta Actin / �-Actin Mouse Monoclonal (ACTB/1109). Inset: PBS instead of primary antibody; secondary only negative control.

Western blot analysis of Raji cell lysate using Beta Actin / �-Actin Mouse Monoclonal (ACTB/1109).

Formalin-fixed, paraffin-embedded human tonsil stained with Beta Actin / �-Actin Mouse Monoclonal (ACTB/1109). Inset: PBS instead of primary antibody; secondary only negative control.

Product Details

Synonyms

A26C1A; A26C1B; ACTB; Actin beta; Actin, cytoplasmic 1, N-terminally processed; Actx; b actin; Beta cytoskeletal actin; Beta-actin; BRWS1; E430023M04Rik; Melanoma X actin; MGC128179; PS1TP5 binding protein 1; PS1TP5BP1

Positive Control

Human myoepithelial cells. HeLa, Jurkat or Raji cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human ACTB protein

Cellular Localization

Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Species Reactivity

Human, Mouse and Rat. Predicted to react in Xenopus, Bovine, Chicken, Horse, Monkey, Pig and Sheep.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

43kDa

Specificity & Comments

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas -Actin and g-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.