|Catalog No||Formulation||Size||Price (USD)|
|7056-MSM1-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|7056-MSM1-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|7056-MSM1-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||7056|
|Human Gene Symbol||THBD|
|Human Chromosome Location||20p11.2|
|Synonyms||AHUS6; BDCA3; CD141; Fetomodulin; Thbd; THPH12; THRM; Thrombomodulin (TM)|
|Immunogen||Recombinant human Thrombomodulin protein fragment (aa69-194) (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG2b, kappa|
|Mol. Weight of Antigen||~100kDa|
|Cellular Localization||Cell Surface & Cytoplasmic|
|Species Reactivity||Human. Others-not known.|
It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Flow Cytometry (0.5-1ug/million cells)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris buffer with1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.