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Home >> Antibodies >> SOX10 (Melanoma Marker)

SOX10 (Melanoma Marker)

Mouse Monoclonal Antibody [Clone SOX10/1074]

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Catalog No Formulation Size Price (USD)  
6663-MSM3-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
6663-MSM3-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
6663-MSM3-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 6663
Human SwissProt P56693
Human Unigene 376984
Human Gene Symbol SOX10
Human Chromosome Location 22q13.1
Synonyms DOM; PCWH; SOX10; SRY (sex determining region Y) box 10; SRY box containing gene 10; SRY related HMG box gene 10; Waardenburg syndrome type 2EÂ(WS2E); WS4; Waardenburg syndrome type 4C (WS4C)Â
Immunogen Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen 49-58kDa
Cellular Localization Nuclear
Species Reactivity Human and Mouse. Others not known.

Specificity & Comments

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately-to-strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Known Applications & Suggested Dilutions

Western Blotting (0.5-1.0ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HepG2 cells. Melanomas, breast carcinomas, gliomas.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Mohamed A, et al. Appl Immunohistochem Mol Morphol. 2013; 21(6):506-10.?
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