|Catalog No||Formulation||Size||Price (USD)|
|2312-MSM1-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|2312-MSM1-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|2312-MSM1-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||2312|
|Human Gene Symbol||FLG|
|Human Chromosome Location||1q21.3|
|Synonyms||ATOD2; Epidermal Filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin|
|Immunogen||Recombinant human Filaggrin protein fragment (aa 998-1104) (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG1|
|Mol. Weight of Antigen||26-45kDa (Processed); 350kDa (Profilaggrin)|
|Species Reactivity||Human. Others-not tested.|
Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
Flow Cytometry (0.5-1ug/million cells)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
U-251-MG cells or Skin
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.