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Catenin, delta-1 (CTNND1) (pTyr96)

Mouse Monoclonal Antibody [Clone 25a]

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Catalog No Formulation Size Price (USD)  
1500-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
1500-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
1500-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
Human Entrez Gene ID 1500 (Human)
Human SwissProt O60716 (Human)
Human Unigene 166011 (Human)
Human Gene Symbol CTNND1
Human Chromosome Location 11q11
Synonyms Cadherin-associated Src substrate (CAS); Catenin (cadherin associated protein) delta 1; Catenin delta-1; CTNND1; p120 catenin; P120 CTN; p120(cas); p120(ctn); P120CAS; P120CTN
Immunogen Mouse p120 Catenin (pY96)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 120kDa
Cellular Localization Cell Surface
Species Reactivity Human and Rat. Others not known.

Specificity & Comments

The membrane associated protein pp120 Src substrate (p120 Catenin, p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src transformed cells or in response to growth factor stimulation. It shares structural similarity with the Drosophila Armadillo protein and the vertebrate beta-catenin and gamma-catenin proteins. Its characteristic Arm domain that is composed of 42-amino acid motif repeats evidences this similarity. In the cell, p120 Catenin is localized to the E-Cadherin/catenins cell adhesion complex. Like beta- and gamma-catenin, p120 Catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain and may similarly interact with other Cadherins. It exists as four isoforms that range in size from 90-115kDa. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered pp120 expression contributes to malignancy due to loss of functional cell adhesions. Multiple tyrosine residues (Y96, Y112, Y228, Y280, Y257, Y291, Y296, and Y302) in p120 Catenin are phosphorylated by Src and these phosphorylations may facilitate interaction with PTP1C/SHP-1 in response to EGF stimulation. Thus, p120 Catenin is an Arm domain protein that interacts with both cell adhesion molecules, such as cadherins and cell signaling molecules, such as PTP1C.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Positive Control

Carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Reynolds, Daniel, McCrea et al.: "Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8333-42, 1994.
  2. Mariner, Anastasiadis, Keilhack et al.: "Identification of Src phosphorylation sites in the catenin p120ctn." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28006-13, 2001
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