|Catalog No||Formulation||Size||Price (USD)|
|1499-RBP1-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|1499-RBP1-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|1499-RBP1-P1ABX||Purified Ab WITHOUT BSA at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||1499|
|Human Gene Symbol||CTNNB1|
|Human Chromosome Location||3p22.1|
|Synonyms||Cadherin associated protein), beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1|
|Immunogen||A synthetic peptide from the middle of beta-Catenin (p120) protein (exact sequence is proprietary)|
|Host / Ig Isotype||Rabbit / IgG, kappa|
|Mol. Weight of Antigen||92kDa|
|Cellular Localization||Cell surface and cytoplasmic|
|Species Reactivity||Human and Mouse. Others-not known.|
Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
Flow Cytometry (0.5-1ug/million cells)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
HeLa or MCF-7 cells. Breast carcinoma
200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.