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Home >> Antibodies >> Catenin, beta (p120)

Catenin, beta (p120)

Mouse Monoclonal Antibody [Clone CTNNB1/1509]

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Catalog No Formulation Size Price (USD)  
1499-MSM9-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
1499-MSM9-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
1499-MSM9-P1ABX Purified Ab WITHOUT BSA at 1.0mg/ml 100 ug 399.00
Human Entrez Gene ID 1499
Human SwissProt P35222
Human Unigene 476018
Human Gene Symbol CTNNB1
Human Chromosome Location 3p22.1
Synonyms Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1
Immunogen Recombinant human full-length beta-Catenin (p120) protein fragment
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 92kDa
Cellular Localization Cell surface and cytoplasmic
Species Reactivity Human, Mouse and Rat. Others-not known.

Specificity & Comments

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tis with1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HeLa or MCF-7 cells. Breast carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Dabbs DJ et. al. Am J Surg Path. 2007;31:427-437.
  2. Sarrio D et. al. Oncogene. 2004;23:3272-3283.
  3. Mastracci TL et. al. Mod Path. 2005;18:741-751.
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