2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Catenin, beta (p120)

Catenin, beta (p120)

Mouse Monoclonal Antibody [Clone 9F2]

pdf icon
Catalog No Formulation Size Price (USD)  
1499-MSM6-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
1499-MSM6-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
1499-MSM6-P1ABX Purified Ab WITHOUT BSA at 1.0mg/ml 100 ug 399.00
Human Entrez Gene ID 1499
Human SwissProt P35222
Human Unigene 476018
Human Gene Symbol CTNNB1
Human Chromosome Location 3p22.1
Synonyms Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1
Immunogen Recombinant chicken ?-catenin
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 92kDa
Cellular Localization Cell surface and cytoplasmic
Species Reactivity Human, Cow, Dog and Chicken. Others-not known.

Specificity & Comments

This MAb recognizes a protein of 92kDa, which is identified as beta-catenin. It shows no cross-reaction with gamma-catenin (also known as plakoglobin). The catenins, alpha, beta and gamma bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Alpha-catenin was initially described as an E-cadherin-associated protein, and has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. Beta-catenin has also been found in complexes with the tumor suppressor protein APC.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Optimal dilution for a specific application should be determined.

Positive Control

A431, A549 or MCF-7 cells. Breast carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Knudsen KA, Soler AP, Johnson KR, Wheelock MJ. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol. 1995; 130(1): 67-77.
  2. Sacco, PA; McGranahan TM; Wheelock, MJ; Johnson, KR. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem 1995, 270: 20201-6.
DOC#01446