|Catalog No||Formulation||Size||Price (USD)|
|1499-MSM5-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|1499-MSM5-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|1499-MSM5-P1ABX||Purified Ab WITHOUT BSA at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||1499|
|Human Gene Symbol||CTNNB1|
|Human Chromosome Location||3p22.1|
|Synonyms||Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1|
|Immunogen||Recombinant chicken ?-catenin|
|Host / Ig Isotype||Mouse / IgG1, kappa|
|Mol. Weight of Antigen||92kDa|
|Cellular Localization||Cell surface and cytoplasmic|
|Species Reactivity||Human, Cow, Dog and Chicken. Others-not known.|
This MAb recognizes a protein of 92kDa, which is identified as beta-catenin. It shows no cross-reaction with gamma-catenin (also known as plakoglobin). The catenins, alpha, beta and gamma bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Alpha-catenin was initially described as an E-cadherin-associated protein, and has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. Beta-catenin has also been found in complexes with the tumor suppressor protein APC.
Flow Cytometry (0.5-1ug/million cells)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
HeLa or MCF-7 cells. Breast carcinoma
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.