|Catalog No||Formulation||Size||Price (USD)|
|1048-MSM10-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|1048-MSM10-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|1048-MSM10-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||1048 & 634|
|Human Gene Symbol||CEACAM1 & CEACAM5|
|Human Chromosome Location||19q13.1-19q13.2|
|Synonyms||Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)|
|Immunogen||Purified human CEA protein|
|Host / Ig Isotype||Mouse / IgG2b, kappa|
|Mol. Weight of Antigen||80-200kDa|
|Cellular Localization||Cytoplasmic and luminal surface|
|Species Reactivity||Human. Others not known.|
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb reacts with nonspecific cross-reacting antigen (NCA). It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
Flow Cytometry (0.5-1ug/million cells)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
MCF7 or 293T cells. Colon carcinoma
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.