2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Carcinoembryonic Antigen (CEA) / CD66

Carcinoembryonic Antigen (CEA) / CD66

Mouse Monoclonal Antibody [Clone C66/1260]

pdf icon
Catalog No Formulation Size Price (USD)  
1048-MSM10-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
1048-MSM10-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
1048-MSM10-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
Human Entrez Gene ID 1048 & 634
Human SwissProt P06731
Human Unigene 709196
Human Gene Symbol CEACAM1 & CEACAM5
Human Chromosome Location 19q13.1-19q13.2
Synonyms Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)
Immunogen Purified human CEA protein
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen 80-200kDa
Cellular Localization Cytoplasmic and luminal surface
Species Reactivity Human. Others not known.

Specificity & Comments

This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb reacts with nonspecific cross-reacting antigen (NCA). It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

MCF7 or 293T cells. Colon carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Muraro R, et. al. Cancer Research, 1985, 45:5769-80.
  2. Siler K, et. al. Biotechnology Therapeutics, 1993, 4(3-4):163-81.
  3. Robbins PF, et. al. International Journal of Cancer, 1993, 53(6):892-7.
DOC#01260