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Home >> Antibodies >> Calnexin (Endoplasmic Reticulum Marker)

Calnexin (Endoplasmic Reticulum Marker)

Mouse Monoclonal Antibody [Clone CANX/1541]

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Catalog No Formulation Size Price (USD)  
821-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
821-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
821-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
Human Entrez Gene ID 821
Human SwissProt P27824
Human Unigene 567968
Human Gene Symbol CANX
Human Chromosome Location 5q35.3
Synonyms Calnexin; CANX; CNX; IP90; Major histocompatibility complex class I antigen-binding protein p88; P90
Immunogen Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen 90kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Does not react with rat. Others not known.

Specificity & Comments

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Known Applications & Suggested Dilutions

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blotting (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Williams, D.B. 1995. Calnexin: a molecular chaperone with a taste for carbohydrate. Biochem. Cell Biol. 73: 123-132.
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