|Catalog No||Formulation||Size||Price (USD)|
|5175-RBM11-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|5175-RBM11-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|5175-RBM11-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||5175|
|Human Unigene||376675 & 514412|
|Human Gene Symbol||PECAM1|
|Human Chromosome Location||17q23.3|
|Synonyms||EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'|
|Immunogen||Human full-length recombinant CD31 protein|
|Host / Ig Isotype||Rabbit / IgG|
|Mol. Weight of Antigen||~100kDa (endothelium) and ~130kDa (platelets)|
|Cellular Localization||Cell Surface and Cytoplasmic|
|Species Reactivity||Human. Others not known.|
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Flow Cytometry (0.5-1ug/million cells)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Jurkat cells. Tonsil or Angiosarcoma.
200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.