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Home >> Antibodies >> CA19-9/Sialyl Lewisa (GI Tumor Marker)

CA19-9/Sialyl Lewisa (GI Tumor Marker)

Mouse Monoclonal Antibody [Clone 121SLE]

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Catalog No Formulation Size Price (USD)  
MSM1-25-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
MSM1-25-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
MSM1-25-P1ABX Purified Ab WITHOUT BSA at 1.0mg/ml 100 ug 399.00
Human Entrez Gene ID Not Known (Human)
Human SwissProt Not Known (Human)
Human Unigene Not Known (Human)
Human Gene Symbol
Human Chromosome Location
Synonyms CA19.9, Ovarian tumor antigen, Pancreatic tumor antigen, Sialyl Lewis a
Immunogen Precipitin lines obtained after immuno-diffusion using MAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le).
Host / Ig Isotype Mouse / IgM, kappa
Mol. Weight of Antigen >400kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Others not known.

Specificity & Comments

CA19-9, a carbohydrate epitope expressed on a high MW (>400kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive Control

Stomach or Colon Carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Blood transfusion and Immunohaematology, Ph. Rouger, D Anstee and Ch. Salmon (Eds). Arnette, France 30 (5): 353-720 (1987).Tumor Biol 19(5): 390420, (1998; TD-6 Workshop Report).
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