|Catalog No||Formulation||Size||Price (USD)|
|604-MSM6-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|604-MSM6-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|604-MSM6-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||604|
|Human Gene Symbol||BCL6|
|Human Chromosome Location||3q27.3|
|Synonyms||B-cell lymphoma 5 protein; B-Cell Lymphoma 6 Protein; BCL5; BCL6; BCL6A; cys his2 zinc finger transcription factor; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc Finger Protein 51 (ZNF51); zinc finger transcription factor BCL6S|
|Immunogen||Recombinant human bcl-6 protein fragment (aa256-389) (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG1|
|Mol. Weight of Antigen||95kDa|
|Species Reactivity||Human. Others not known.|
Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ( L&H ) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
Flow Cytometry (0.5-1ug/million cells)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Raji or Ramos cells. Tonsil or Hodgkin s lymphoma.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.