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Home >> Antibodies >> Bcl-2 (Apoptosis & Follicular Lymphoma Marker)

Bcl-2 (Apoptosis & Follicular Lymphoma Marker)

Mouse Monoclonal Antibody [Clone 100/D5]

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Catalog No Formulation Size Price (USD)  
596-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
596-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
596-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
596-MSM1-IHC7 Prediluted Ab for IHC 7.0 ml 329.00
Human Entrez Gene ID 596
Human SwissProt P10415
Human Unigene 150749
Human Gene Symbol BCL2
Human Chromosome Location 18q21.33
Synonyms Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2
Immunogen A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 25-26kDa
Cellular Localization Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Species Reactivity Human, Does not react with mouse and rat. Others-not known.

Specificity & Comments

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 α oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 α oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 α protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Pezzella F et. al.. American Journal of Pathology, 1990, 137(2):225-32.
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