|Catalog No||Formulation||Size||Price (USD)|
|672-MSM1-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|672-MSM1-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|672-MSM1-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||672|
|Human Gene Symbol||BRCA1|
|Human Chromosome Location||17q21|
|Synonyms||BRCA1; Breast and ovarian cancer susceptibility protein 1; Breast Cancer 1 Early Onset; Breast cancer type 1 susceptibility protein; BROVCA1; IRIS; PNCA4; PPP1R53; Protein phosphatase 1 regulatory subunit 53; PSCP; RING finger protein 53; RNF53|
|Immunogen||Recombinant human BRCA1 protein fragment (aa445-620) (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG1|
|Mol. Weight of Antigen||220kDa|
|Cellular Localization||Cytoplasmic and Nuclear|
|Species Reactivity||Human. Others-not tested.|
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.
Flow Cytometry (0.5-1ug/million cells)
Immunohistology (Formalin-fixed) (5-10ug/ml for 60 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.