|Catalog No||Formulation||Size||Price (USD)|
|12-MSM1-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|12-MSM1-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|12-MSM1-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||12|
|Human Unigene||534293, 710488|
|Human Gene Symbol||SERPINA3|
|Human Chromosome Location||14q32.1|
|Synonyms||SERPINA3; AACT; ACT; Alpha-1-antichymotrypsin; Antichymotrypsin; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; Growth inhibiting protein 24; Growth inhibiting protein 25; Serine (or cysteine) proteinase inhibitor clade A member 3; Serine proteinase inhibitor clade A member 3; Serpin A3; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3|
|Immunogen||Recombinant human Antichymotrypsin (AACT) protein fragment (aa49-187) (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG1|
|Mol. Weight of Antigen||65-76kDa|
|Species Reactivity||Human. Others-not known.|
It recognizes a protein of 65-76kDa, which is identified antichymotrypsin (AACT). AACT is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of α 1-antitrypsin (AAT), with which it shares nucleic acid and protein sequence homology. Both are major acute phase reactants; their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. AACT antibody reacts with histiocytes and histiocytic neoplasms. It is widely used to identify histiocytes and tumors derived from them. Acinar tumors of the pancreas and salivary gland may also exhibit AACT positivity.
Flow Cytometry (0.5-1ug/million cells)
western Blotting (0.5-1.0ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
HeLa Cells. Tonsil, Pancreas or Histiocytoma.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.