|Catalog No||Formulation||Size||Price (USD)|
|250-MSM5-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|250-MSM5-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|250-MSM5-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|250-MSM5-IHC7||Prediluted Ab for IHC||7.0 ml||329.00|
|Human Entrez Gene ID||250|
|Human Gene Symbol||ALPP|
|Human Chromosome Location||2q37.1|
|Synonyms||Alkaline phosphatase placental type; Alkaline phosphatase Regan Isozyme; ALP; Alp1; ALPP; Germ-cell alkaline phosphatase; nagao Isozyme; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP-1; PLAP1|
|Immunogen||Purified human PLAP protein|
|Host / Ig Isotype||Mouse / IgG2b, kappa|
|Mol. Weight of Antigen||70kDa|
|Cellular Localization||Cytoplasmic and cell surface|
|Species Reactivity||Human, Others not known.|
Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunohistology (Frozen & Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)
(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.)
Optimal dilution for a specific application should be determined.
HepG2 cells. Placenta or seminoma
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.