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Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)

Mouse Monoclonal Antibody [Clone ALPP/238]

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Catalog No Formulation Size Price (USD)  
250-MSM2-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
250-MSM2-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
250-MSM2-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
Human Entrez Gene ID 250
Human SwissProt P05187
Human Unigene 284255
Human Gene Symbol ALPP
Human Chromosome Location 2q37.1
Synonyms Alkaline phosphatase placental type; Alkaline phosphatase Regan Isozyme; ALP; Alp1; ALPP; Germ-cell alkaline phosphatase; nagao Isozyme; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP-1; PLAP1
Immunogen Recombinant full-length human ALPP protein
Host / Ig Isotype Mouse / IgG2a, kappa
Mol. Weight of Antigen 70kDa
Cellular Localization Cytoplasmic and cell surface
Species Reactivity Human, Others not known.

Specificity & Comments

PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This MAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP as to AP from normal human testis. This MAb can be used both as coating as well as tracer antibody in the same ELISA to detect PLAP in serum of S, F, FS and I phenotypes.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Frozen) (1-2ug/ml for 30 minutes at RT)
Optimal dilution for a specific application should be determined.

Positive Control

HepG2 cells. Placenta or seminoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Millan J.L. et. al., Antigenic determinants of human placental and testicular alkaline phosphatase as mapped by monoclonal antibodies. Eur. J. Biochem. 136: 1-12, (1983).
  2. Riklund K.E. et. al., Experimental radio-immunotherapy of HeLa tumors in nude mice with 131I-labeled monoclonal antibodies. Anticancer Research, 1990, 10:379-84.
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