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Home >> Antibodies >> Actin, Smooth Muscle (Leiomyosarcoma Marker)

Actin, Smooth Muscle (Leiomyosarcoma Marker)

Mouse Monoclonal Antibody [Clone 1A4; same as asm-1]

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Catalog No Formulation Size Price (USD)  
59-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
59-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
59-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
59-MSM1-IHC7 Prediluted Ab for IHC 7.0 ml 329.00
Human Entrez Gene ID 59
Human SwissProt P62736
Human Unigene 500483
Human Gene Symbol ACTA2
Human Chromosome Location 10q23.31
Synonyms ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46
Immunogen N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
Host / Ig Isotype Mouse / IgG2a, kappa
Mol. Weight of Antigen 42kDa
Cellular Localization Cytoplasmic
Species Reactivity Human, Baboon, Monkey, Cow, Pig, Sheep, Goat, Cat, Dog, Rabbit, Mouse, Rat, Guinea pig and Chicken. Others-not known.

Specificity & Comments

Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Skalli O et. al. Journal of Cell Biology, 1986, 103:2787-96.
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