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Home >> Antibodies >> Actin, Muscle Specific (Muscle Cell Marker)

Actin, Muscle Specific (Muscle Cell Marker)

Mouse Monoclonal Antibody [Clone HHF35 + MSA/953]

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Catalog No Formulation Size Price (USD)  
MSM3-1013-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
MSM3-1013-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
MSM3-1013-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
MSM3-1013-IHC7 Prediluted Ab for IHC 7.0 ml 329.00
Human Entrez Gene ID 58, 59, 70
Human SwissProt P62736, P68032, P68133
Human Unigene 118127, 1288, 500483
Human Gene Symbol ACTA1 (Skeletal); ACTA2 (Smooth); ACTG2 (Smooth)
Human Chromosome Location 1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
Synonyms ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1
Immunogen Recombinant human actin fragment
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen Multiple
Cellular Localization Cytoplasmic
Species Reactivity Human, Rabbit and Rat. Others-not known.

Specificity & Comments

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Muscle or sarcoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Barkalow, K. and Hartwig, J.H. 1995. Actin cytoskeleton. Setting the pace of cell movement. Curr. Biol. 5: 1000-1002.