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Home >> Antibodies >> AMACR / p504S (Prostate Cancer Marker)

AMACR / p504S (Prostate Cancer Marker)

Recombinant Rabbit Monoclonal Antibody [Clone 13H4]

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Catalog No Formulation Size Price (USD)  
23600-RBM1-CS0 Culture Supernatant 0.1 ml 199.00
23600-RBM1-CS1 Culture Supernatant 0.5 ml 379.00
23600-RBM1-CS2 Culture Supernatant 1.0 ml 459.00
23600-RBM1-IHC7 Prediluted Ab for IHC 7.0 ml 329.00
Human Entrez Gene ID 23600
Human SwissProt Q9UHK6
Human Unigene 508343
Human Gene Symbol AMACR
Human Chromosome Location 5p13.3
Synonyms Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM
Immunogen Full length human recombinant AMACR protein
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 54kDa
Cellular Localization Cytoplasmic
Species Reactivity Human, Others not known.

Specificity & Comments

This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Known Applications & Suggested Dilutions

Immunohistology (Formalin-fixed) (1:50-1:100 for 30-60 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HEK cells or Prostate Adenocarcinoma

Supplied As

Culture Supernatant with 0.05% Azide.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Xu J et. al. Canc Res. 2000; 60:1677
  2. Jiang Z et. al. Human Pathology. 2003; 34(8):792
  3. Jiang Z et. al. Am J Surg Pathol. 2001; 25(11):1397
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