|Catalog No||Formulation||Size||Price (USD)|
|174-MSM4-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|174-MSM4-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|174-MSM4-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|174-MSM4-IHC7||Prediluted Ab for IHC||7.0 ml||329.00|
|Human Entrez Gene ID||174|
|Human Gene Symbol||AFP|
|Human Chromosome Location||4q13.3|
|Synonyms||Alpha fetoglobulin; FETA; HPAFP|
|Immunogen||Recombinant full-length human Alpha fetoprotein|
|Host / Ig Isotype||Mouse / IgG1, kappa|
|Mol. Weight of Antigen||70kDa|
|Species Reactivity||Human, Others-not known.|
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. The yolk sac and the liver produce AFP during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spinal bifida and anencephaly.
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Hep-G2 cells. Fetal liver or hepatocellular carcinoma
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.