2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker)

AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker)

Mouse Monoclonal Antibody [Clone D10]

pdf icon
Catalog No Formulation Size Price (USD)  
174-MSM3-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
174-MSM3-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
174-MSM3-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
Human Entrez Gene ID 174
Human SwissProt P02771
Human Unigene 518808
Human Gene Symbol AFP
Human Chromosome Location 4q13.3
Synonyms Alpha fetoglobulin; FETA; HPAFP
Immunogen Alpha fetoprotein (AFP) purified from serum of a hepatoma patient
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 70kDa
Cellular Localization Cytoplasmic
Species Reactivity Human, It does not react with cow, Dog, Mouse, and rat.

Specificity & Comments

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop and assigned by K. Nustad to group D of a cluster of 6 major epitopes of human alpha fetoprotein). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Optimal dilution for a specific application should be determined.

Positive Control

Hep-G2 cells. Fetal liver or hepatocellular carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Tsung K., et al.Milunsky A., Alpert E. Derivation and characterization of a monoclonal hybridoma antibody specific for human alpha-fetoprotein. J. Immunol. Methods 39: 363-368, (1980)
  2. Michell B., Fiebach H., Karsten U., Goussev A.I., Yazova A.K., Knopp J. Monoclonal antibodies to different epitopes of human alpha-fetoprotein (AFP). Eur. J. Cancer Clin. Oncol. 19:1239-1246, (1983).
  3. Yazova A.K., Goussev A.I., Poltorania V.S., Yakimenko E.F., Human alpha- fetoprotein epitopes as revealed by monoclonal antibodies. Immunol. Lett. 25: 325-330, (1990).
  4. Nustad K., Paus E., Kierulf B., Bormer O.P. Specificity and affinity of 30 monoclonal antibodies against alpha-fetoprotein. Tumor Biol 19: 293 -300, (1998).
  5. Yakimenko E.F., Karamova E.R., Goussev A.I., Hilgers J., Abelev G.I., Yazova A.K.: Epitope mapping of human alpha-fetoprotein. Tumor Biol 19: 301309, (1998).
DOC#00331