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Home >> Antibodies >> AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker)

AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker)

Mouse Monoclonal Antibody [Clone C2 + C3 + MBS-12]

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Catalog No Formulation Size Price (USD)  
174-MSM2-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
174-MSM2-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
174-MSM2-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
174-MSM2-IHC7 Prediluted Ab for IHC 7.0 ml 329.00
Human Entrez Gene ID 174
Human SwissProt P02771
Human Unigene 518808
Human Gene Symbol AFP
Human Chromosome Location 4q13.3
Synonyms Alpha fetoglobulin; FETA; HPAFP
Immunogen Recombinant full-length human Alpha fetoprotein
Host / Ig Isotype Mouse / IgG's
Mol. Weight of Antigen 70kDa
Cellular Localization Cytoplasmic
Species Reactivity Human, Others not known.

Specificity & Comments

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HepG2 cells. Fetal liver or hepatocellular carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Lafuste, P., et al. 2002. α-fetoprotein gene expression in early and full- term human trophoblast. Placenta 23: 600-612.
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