IHC Protocol

RE-Hydration Of Tissue Slides:

  • Label the tissue slides according to the protocol arrange them well in slide racks.
  • Bake slides in an oven at 60°C for 15 mins or until the paraffin on the tissue loosens and forms droplets.
  • Deparaffinize and rehydrate tissue sections by immersing slides through following series of solutions.
    Slide Brite 3 changes, 5 minutes each. (Slide Brite is a Xylene substitute)
    100% ethanol, 2 changes each
    95% ethanol, 2 changes 5 minutes each
    80% ethanol, 5 minutes
    50% ethanol, 5 minutes
    Distilled water, 2 changes 5 minutes each
    Hydrogen Peroxide 3%, 2 changes 5 minutes each.

Retrieval Of Antigen:

  • Antigen retrieval using BioCare’s Decloacker. Boil sections at 95°C for 45 minutes using Tris-EDTA (10mM Tris with 1mM EDTA, pH9.0) OR Citrate buffer (10mM Citrate pH6.0).


  • Use hydrophobic marker to create a barrier above and below tissue sections. Care must be taken in this step because sometimes marker can cause tissue dry if marking is near to the tissue. So, avoid close marking in this step.
  • Wash section twice, 3 times each in PBS.
  • Incubate primary antibody for 30 minutes.
  • Wash sections twice with PBS , 3-5 minutes each.
  • Incubate for 30 minutes with secondary antibody, goat anti-mouse / goat anti-rabbit-HRP polymer.
  • DAB 3 to 5 minutes Preparation of DAB: Take 950ul of DAB substrate add 500ul of DAB chromogen (ScyTek Laboratories). Mix well and use immediately.
  • Wash sections with PBS for 3-5 minutes, final wash should be DW.
  • Staining with Hematoxylin and bluing solutions Dip slide in hematoxylin 3-5 times and wash slides in tap water for 3 times. Dip slides in bluing solutions and wash slides in tap water for 3 times.
  • Observe cells under a microscope and find out the cellular localization in tissues.

Safety Data Sheet

NeoBiotechnologies Receives 2023 Best of Union City Award

Press Release


NeoBiotechnologies Receives 2023 Best of Union City Award

Union City Award Program Honors the Achievement

UNION CITY September 5, 2023 — NeoBiotechnologies has been selected for the 2023 Best of Union City Award in the Biotechnology Company category by the Union City Award Program.

Each year, the Union City Award Program identifies companies that we believe have achieved exceptional marketing success in their local community and business category. These are local companies that enhance the positive image of small business through service to their customers and our community. These exceptional companies help make the Union City area a great place to live, work and play.

Various sources of information were gathered and analyzed to choose the winners in each category. The 2023 Union City Award Program focuses on quality, not quantity. Winners are determined based on the information gathered both internally by the Union City Award Program and data provided by third parties.

About Union City Award Program

The Union City Award Program is an annual awards program honoring the achievements and accomplishments of local businesses throughout the Union City area. Recognition is given to those companies that have shown the ability to use their best practices and implemented programs to generate competitive advantages and long-term value.

The Union City Award Program was established to recognize the best of local businesses in our community. Our organization works exclusively with local business owners, trade groups, professional associations and other business advertising and marketing groups. Our mission is to recognize the small business community’s contributions to the U.S. economy.

SOURCE: Union City Award Program

Union City Award Program
Email: PublicRelations@citiesawardcompany.com
URL: https://www.citiesawardcompany.com

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