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Home >> Antibodies >> TSH-Receptor, B-Chain (Thyroid Marker)

TSH-Receptor, B-Chain (Thyroid Marker)

Mouse Monoclonal Antibody [Clone TSHRB/1405]

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Catalog No Format Size Price (USD)
7253-MSM5-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
7253-MSM5-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
7253-MSM5-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
7253-MSM5-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
7253-MSM5-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 7253
Human SwissProt P16473
Human Unigene 160411
Human Gene Symbol TSHR
Human Chromosome Location 14q31
Synonyms CHNG1; hTSHRI; LGR3; Thyroid-stimulating hormone receptor; Thyrotropin receptor; Thyrotropin receptor I; TSHR
Immunogen Recombinant human TSHR, B-Chain protein
Host / Ig Isotype Mouse / IgG2a
Mol. Weight of Antigen 42kDa
Cellular Localization Cell Surface & Cytoplasmic
Species Reactivity Human. Others not known.
    

Specificity & Comments

Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Grave s disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

293T Cells. Thyroid gland

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. sTanaka, K., et al. 1999. Subunit structure of thyrotrophin receptors expressed on the cell surface. J. Biol. Chem. 274: 33979-33984.
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