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Home >> Antibodies >> MART-1 / Melan-A / MLANA (Melanoma Marker)

MART-1 / Melan-A / MLANA (Melanoma Marker)

Recombinant Rabbit Monoclonal Antibody [Clone MLANA/1409R]

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Catalog No Format Size Price (USD)
2315-RBM9-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
2315-RBM9-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
2315-RBM9-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
2315-RBM9-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
2315-RBM9-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 2315
Human SwissProt Q16655
Human Unigene 154069
Human Gene Symbol MLANA
Human Chromosome Location 9p24.1
Synonyms Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
Immunogen Recombinant human full-length MLANA protein
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 20-22kDa (doublet)
Cellular Localization Cytoplasmic
Species Reactivity Human, Mouse and Rat. Others-not tested.
    

Specificity & Comments

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1.0ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas

Supplied As

200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.
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