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Home >> Antibodies >> GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker)

GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker)

Mouse Monoclonal Antibody [Clone PIP/1571]

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Catalog No Format Size Price (USD)
5304-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
5304-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
5304-MSM1-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
5304-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
5304-MSM1-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 5304
Human SwissProt P12273
Human Unigene 99949
Human Gene Symbol PIP
Human Chromosome Location 7q34
Synonyms BRST-2; GCDFP-15; gp17; GPIP4; Gross cystic disease fluid protein 15; Prolactin-induced protein (PIP); Prolactin-inducible protein (PIP); Secretory actin-binding protein (SABP)
Immunogen Recombinant human GCDFP-15 protein fragment (aa 41-146) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2a, kappa
Mol. Weight of Antigen 15kDa
Cellular Localization Cytoplasmic (Golgi bodies) and Secreted
Species Reactivity Human. Others-not known.
         

Specificity & Comments

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris buffer with1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HepG2 Cells. Breast or Pancreas.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Wick MR, Lillemoe TJ, Copland GT, Swanson PE, Manivel JC, Kiang DT. Gross cystic disease fluid protein-15 as a marker for breast cancer: immunohistochemical analysis of 690 human neoplasms and comparison with alpha-lactalbumin. Hum Pathol. 1989 Mar;20(3):281-7.
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