2 Union Square, Union City, CA 94587 contact@NeoBiotechnologies.com 510-376-5603

Home >> Antibodies >> Filaggrin (Keratinocyte Differentiation Marker)

Filaggrin (Keratinocyte Differentiation Marker)

Mouse Monoclonal Antibody [Clone FLG/1561]

PDF Version  Printable Version
Catalog No Format Size Price (USD)
2312-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
2312-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
2312-MSM1-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
2312-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
2312-MSM1-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 2312
Human SwissProt P20930
Human Unigene 654510
Human Gene Symbol FLG
Human Chromosome Location 1q21.3
Synonyms ATOD2; Epidermal Filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin
Immunogen Recombinant human Filaggrin protein fragment (aa 998-1104) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1
Mol. Weight of Antigen 26-45kDa (Processed); 350kDa (Profilaggrin)
Cellular Localization Cytoplasmic
Species Reactivity Human. Others-not tested.
    

Specificity & Comments

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

U-251-MG cells or Skin

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Gan, S.Q., et al. 1990. Organization, structure, and polymorphisms of the human profilaggrin gene. Biochemistry 29: 9432-9440.
DOC#01561