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Home >> Antibodies >> FOXA1 / HNF3A

FOXA1 / HNF3A

Mouse Monoclonal Antibody [Clone FOXA1/1515]

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Catalog No Format Size Price (USD)
3169-MSM5-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
3169-MSM5-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
3169-MSM5-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
3169-MSM5-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
3169-MSM5-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 3169
Human SwissProt P55317
Human Unigene 163484
Human Gene Symbol FOXA1
Human Chromosome Location 14q21.1
Synonyms Forkhead box protein A1 (FOXA1); Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A); Transcription factor 3A (TCF-3A or TCF3A)
Immunogen Recombinant human FOXA1 protein fragment (aa467-614) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 79kDa
Cellular Localization Nuclear
Species Reactivity Human. Does not react with rat. Others not known.
         

Specificity & Comments

The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. sAndre Albergaria, Joana Paredes, Barbara Sousa, Fernanda Milanezi, Vitor Carneiro, Joana Bastos, Sandra Costa, Daniella Vieira, Nair Lopes, Eric W Lam, Nuno Lunet and Fernando Schmitt. Expression of FOXA1 and GATA-3 in breast cancer: the prognostic significance in hormone receptor-negative tumours. Breast Cancer Research 2009, 11:R40.
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